Measurement of TGF-beta in biological fluids.

H. Hale-Donze, National Institute of Dental and Craniofacial Research (NIDCR)
S. M. Wahl, National Institute of Dental and Craniofacial Research (NIDCR)
R. J. Jackson, National Institute of Dental and Craniofacial Research (NIDCR)
J. R. McGhee, National Institute of Dental and Craniofacial Research (NIDCR)

Abstract

Transforming growth factor (TGF-beta) is an immunoregulatory cytokine produced by many different cell types, including lymphocytes and monocytes-macrophages. Two assays are described for the quantitation of TGF-beta activity in supernatant fluids. The bioassay for TGF-beta is based on the potent ability of TGF-beta to suppress lymphocyte proliferation. The radioreceptor assay is based on the ability of TGF-beta-containing supernatant fluids to inhibit the binding of radiolabeled TGF-beta to its cellular receptor. Two support protocols describe acid-activation and neutralization of TGF-beta.