"Monitoring the Photocleaving Dynamics of Colloidal MicroRNA-Functional" by Raju R. Kumal, Corey R. Landry et al.

Faculty Publications

Title

Monitoring the Photocleaving Dynamics of Colloidal MicroRNA-Functionalized Gold Nanoparticles Using Second Harmonic Generation

Authors

Raju R. Kumal, Department of Chemistry and ‡Department of Biological, Agricultural Engineering, Louisiana State University , Baton Rouge, Louisiana 70803, United States.
Corey R. Landry, Department of Chemistry and ‡Department of Biological, Agricultural Engineering, Louisiana State University , Baton Rouge, Louisiana 70803, United States.
Mohammad Abu-Laban, Department of Chemistry and ‡Department of Biological, Agricultural Engineering, Louisiana State University , Baton Rouge, Louisiana 70803, United States.
Daniel J. Hayes, Department of Chemistry and ‡Department of Biological, Agricultural Engineering, Louisiana State University , Baton Rouge, Louisiana 70803, United States.
Louis H. Haber, Department of Chemistry and ‡Department of Biological, Agricultural Engineering, Louisiana State University , Baton Rouge, Louisiana 70803, United States.

Document Type

Article

Publication Date

9-15-2015

Abstract

Photoactivated drug delivery systems using gold nanoparticles provide the promise of spatiotemporal control of delivery that is crucial for applications ranging from regenerative medicine to cancer therapy. In this study, we use second harmonic generation (SHG) spectroscopy to monitor the light-activated controlled release of oligonucleotides from the surface of colloidal gold nanoparticles. MicroRNA is functionalized to spherical gold nanoparticles using a nitrobenzyl linker that undergoes photocleaving upon ultraviolet irradiation. The SHG signal generated from the colloidal nanoparticle sample is shown to be a sensitive probe for monitoring the photocleaving dynamics in real time. The photocleaving irradiation wavelength is scanned to show maximum efficiency on resonance at 365 nm, and the kinetics are investigated at varying irradiation powers to demonstrate that the nitrobenzyl photocleaving is a one-photon process. Additional characterization methods including electrophoretic mobility measurements, extinction spectroscopy, and fluorimetry are used to verify the SHG results, leading to a better understanding of the photocleaving dynamics for this model oligonucleotide therapeutic delivery system.

Publication Source (Journal or Book title)

Langmuir : the ACS journal of surfaces and colloids

First Page

9983

Last Page

Recommended Citation

Kumal, R. R., Landry, C. R., Abu-Laban, M., Hayes, D. J., & Haber, L. H. (2015). Monitoring the Photocleaving Dynamics of Colloidal MicroRNA-Functionalized Gold Nanoparticles Using Second Harmonic Generation. Langmuir : the ACS journal of surfaces and colloids, 31 (36), 9983-90. https://doi.org/10.1021/acs.langmuir.5b02199

Link to Full Text

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