"In vitro characterization of polyesters of aconitic acid, glycerol, an" by Akanksha Kanitkar, Cong Chen et al.

Faculty Publications

Title

In vitro characterization of polyesters of aconitic acid, glycerol, and cinnamic acid for bone tissue engineering

Authors

Akanksha Kanitkar, Audubon Sugar Institute, Louisiana State University Agricultural Center, St. Gabriel, LA, USA Department of Biological and Agricultural Engineering, Louisiana State University and Agricultural Center, Baton Rouge, LA, USA.
Cong Chen, Department of Biological and Agricultural Engineering, Louisiana State University and Agricultural Center, Baton Rouge, LA, USA.
Mollie Smoak, Department of Biological and Agricultural Engineering, Louisiana State University and Agricultural Center, Baton Rouge, LA, USA.
Katie Hogan, Department of Biological and Agricultural Engineering, Louisiana State University and Agricultural Center, Baton Rouge, LA, USA.
Thomas Scherr, Cain Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA, USA.
Giovanna Aita, Audubon Sugar Institute, Louisiana State University Agricultural Center, St. Gabriel, LA, USA GAita@agcenter.lsu.edu.
Daniel Hayes, Department of Biological and Agricultural Engineering, Louisiana State University and Agricultural Center, Baton Rouge, LA, USA.

Document Type

Article

Publication Date

3-1-2015

Abstract

In this study, a novel class of polyesters of glycerol, aconitic acid, and cinnamic acid were synthesized along with their hydroxyapatite (HA) composites, and studied for their potential application in bone defect repair. An osteogenic study was conducted with human adipose derived mesenchymal stem cells (hASCs) to determine the osteoinductive ability of aconitic acid-glycerol (AG) polyesters, AG:HA (80:20), aconitic acid-glycerol-cinnamic acid (AGC) polyesters, and AGC:HA (80:20) to serve as bone scaffolds. The results indicate that AGC scaffolds have the highest mechanical strength in comparison to AG, AG:HA (80:20), and AGC:HA (80:20) scaffolds due to its low porosity. It was determined by cytotoxicity and osteogenesis experiments that hASCs cultured for 21 days on AG:HA (80:20) scaffolds in stromal medium exhibited a greater number of live cells than control PCL:HA composites. Moreover, hASCs cultured on foamed AG:HA (80:20) scaffolds resulted in the highest levels of mineralization, increased alkaline phosphatase (ALP) expression, and the greatest osteocalcin (OCN) expression after 21 days. Overall, AG:HA (100:0 and 80:20) scaffolds had higher mechanical strength and cytocompatibility than the PCL:HA control. In vitro osteogenic study demonstrated that AG:HA (100:0 and 80:20) synthesized using sugarcane industry by-products hold potential as scaffolds for bone tissue engineering applications.

Publication Source (Journal or Book title)

Journal of biomaterials applications

First Page

1075

Last Page

Recommended Citation

Kanitkar, A., Chen, C., Smoak, M., Hogan, K., Scherr, T., Aita, G., & Hayes, D. (2015). In vitro characterization of polyesters of aconitic acid, glycerol, and cinnamic acid for bone tissue engineering. Journal of biomaterials applications, 29 (8), 1075-85. https://doi.org/10.1177/0885328214553961

Link to Full Text

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