An agarose gel electrophoretic method for analysis of sulfated glycosaminoglycans of cultured cells
Document Type
Article
Publication Date
1-1-1978
Abstract
Agarose disc gel electrophoresis has been adapted to achieve the separation of the major sulfated glycosaminoglycans produced by cells in culture. By use of buffers containing barium ion, mixtures of chondroitin sulfate, dermatan sulfate, and heparan sulfate are well resolved into discrete bands. The technique can be used preparatively as well as analytically to separate quantities of glycosaminoglycans up to a milligram in a 6-mm diameter gel. © 1978.
Publication Source (Journal or Book title)
Analytical Biochemistry
First Page
464
Last Page
472
Recommended Citation
Funderburgh, J., & Chandler, J. (1978). An agarose gel electrophoretic method for analysis of sulfated glycosaminoglycans of cultured cells. Analytical Biochemistry, 91 (2), 464-472. https://doi.org/10.1016/0003-2697(78)90532-8
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