Optimum thawing temperature and time for bovine semen processed by three methods and packaged in 1 ml ampules

Document Type

Article

Publication Date

1-1-1983

Abstract

A central composite rotatable design was used to determine optimum temperature and time for thawing semen which was processed in egg yolk-tris-glycerol (ETG), egg yolk-citrate-glycerol (ECG) and skim milk-glycerol (SMG) diluents. Percentage of progressive motility (PPM) and percentage of intact acrosomes (PIA) immediately after thawing (0-hr) and after 3-hr incubation at 37°C were measured. The optimum conditions for temperature and time (C/sec) to sustain maximal PIA response after 3-hr incubation (PIA) were: ETG 42° 121, ECG 33° 175, and SMG 38° 128. Average conditions predicted to be "shared" by all three processing methods were 35°C for 150 sec. Final seminal temperature (FST) was measured at each of these points for ETG, ECG and SMG. Each of the above conditions has a FST above 30°C. A strip plot design was used to compare the optimum and predicted "shared" ( 35°C 150 sec) conditions with recommended method ( 35°C 65 sec) presently in use, denoted as "present". The predicted "shared" thawing conditions resulted in greater (P<0.01) cell viability (PPM and PIA) than did the "present" thawing method both before and after 3-hr incubation. The viability of semen thawed by the predicted "shared" method did not differ (P<0.05) from that resulting from the optimum thawing condition for each processing method (ETG, ECG and SMG) at 0-hr or 3-hr incubation. Cell viability of skim milk-glycerol processed semen degraded faster over the 3-hr incubation period that did that of ETG or ECG processed semen. © 1983.

Publication Source (Journal or Book title)

Theriogenology

First Page

201

Last Page

212

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