In vivo induction of Ia expression in murine cornea after intravitreal injection of interferon-γ

Document Type

Article

Publication Date

1-1-1990

Abstract

Intravitreal injection of interferon gamma (IFN-γ) induces increased expression of Class II major histocompatibility complex (Ia) antigen expression on corneal endothelial cells and stromal fibroblasts. In contrast, IFN-γ has no detectable effect on Ia antigen expression in epithelium. Induction of Ia antigen expression was rapid with increases detectable as early as 6-12 hours after a single injection of 1 x 105 units. Expression peaked at 24-48 hours and decreased to background levels by 120 hours. The Ia antigen expression increased in a dose-dependent manner, and IFN-γ treatment also induced the synthesis of increased amounts of a 65-kilodalton (kD) protein in the cornea. Increased levels of this 65-kd protein are seen as early as 12 hours after treatment and can be induced with as little as 1 x 102 units of IFN-γ. The function of the 65-kd protein is unknown. This model should be useful in studies on in vivo modulation of Ia antigen expression.

Publication Source (Journal or Book title)

Investigative Ophthalmology and Visual Science

First Page

2248

Last Page

2253

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