Ivermectin-dependent attachment of neutrophils and peripheral blood mononuclear cells to Dirofilaria immitis microfilariae in vitro
Document Type
Article
Publication Date
11-15-2014
Abstract
The macrocyclic lactones are the only anthelmintics used to prevent heartworm disease, but it is very difficult to reproduce their in vivo efficacy against Dirofilaria immitis larvae in experiments in vitro. These assays typically measure motility, suggesting that paralysis is not the mode of action of the macrocyclic lactones against D. immitis. We isolated peripheral blood mononuclear cells (PBMC) and neutrophils from uninfected dogs and measured their adherence to D. immitis microfilariae in the presence of varying concentrations of ivermectin. We found that adherence of PBMC to the microfilariae was increased in the presence of ivermectin concentrations ≥100. nM and adherence of neutrophils was increased in drug concentrations ≥10. nM. Up to 50% of microfilariae had adherent PBMC in the presence of the drug, and binding was maximal after 40. h incubation. Neutrophil adherence was maximal after 16. h, with approximately 20% of the microfilariae having at least one cell adhered to them. Adherent neutrophils showed morphological evidence of activation. These results are consistent with a model in which the macrocyclic lactones interfere with the parasites ability to evade the host's innate immune system.
Publication Source (Journal or Book title)
Veterinary Parasitology
First Page
38
Last Page
42
Recommended Citation
Vatta, A., Dzimianski, M., Storey, B., Camus, M., Moorhead, A., Kaplan, R., & Wolstenholme, A. (2014). Ivermectin-dependent attachment of neutrophils and peripheral blood mononuclear cells to Dirofilaria immitis microfilariae in vitro. Veterinary Parasitology, 206 (1-2), 38-42. https://doi.org/10.1016/j.vetpar.2014.02.004