Document Type
Article
Publication Date
5-4-2023
Abstract
Understanding the mechanisms of blastocyst formation and implantation is critical for improving farm animal reproduction but is hampered by a limited supply of embryos. Here, we developed an efficient method to generate bovine blastocyst-like structures (termed blastoids) via assembling bovine trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, in vitro growth, and the ability to elicit maternal recognition of pregnancy following transfer to recipient cows. Bovine blastoids represent an accessible in vitro model for studying embryogenesis and improving reproductive efficiency in livestock species.
Publication Source (Journal or Book title)
Cell Stem Cell
First Page
611
Last Page
616.e7
Recommended Citation
Pinzón-Arteaga, C., Wang, Y., Wei, Y., Ribeiro Orsi, A., Li, L., Scatolin, G., Liu, L., Sakurai, M., Ye, J., Hao Ming., Yu, L., Li, B., Jiang, Z., & Wu, J. (2023). Bovine blastocyst-like structures derived from stem cell cultures. Cell Stem Cell, 30 (5), 611-616.e7. https://doi.org/10.1016/j.stem.2023.04.003