Establishment of bovine trophoblast stem cells

Authors

Yinjuan Wang, LSU Agricultural Center
Hao Ming, LSU Agricultural Center
Leqian Yu, UT Southwestern Medical Center
Jie Li, BGI-Shenzhen
Linkai Zhu, University of Florida
Hai Xi Sun, BGI-Shenzhen
Carlos A. Pinzon-Arteaga, UT Southwestern Medical Center
Jun Wu, UT Southwestern Medical Center
Zongliang Jiang, LSU Agricultural Center

Document Type

Article

Publication Date

5-30-2023

Abstract

Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.

Publication Source (Journal or Book title)

Cell Reports

Recommended Citation

Wang, Y., Ming, H., Yu, L., Li, J., Zhu, L., Sun, H., Pinzon-Arteaga, C., Wu, J., & Jiang, Z. (2023). Establishment of bovine trophoblast stem cells. Cell Reports, 42 (5) https://doi.org/10.1016/j.celrep.2023.112439