Document Type
Article
Publication Date
12-15-2023
Abstract
Here, we present a protocol to isolate progenitor cells from mouse epididymal visceral adipose tissue and construct bulk RNA and assay for transposase-accessible chromatin with sequencing (ATAC-seq) libraries. We describe steps for adipose tissue collection, cell isolation, and cell staining and sorting. We then detail procedures for both ATAC-seq and RNA sequencing library construction. This protocol can also be applied to other tissues and cell types directly or with minor modifications. For complete details on the use and execution of this protocol, please refer to Liu et al. (2023).1
Publication Source (Journal or Book title)
STAR Protocols
Recommended Citation
Liu, Q., Li, C., Li, Y., Wang, L., Zhang, X., Deng, B., Gao, P., Shiri, M., Alkaifi, F., Zhao, J., Stephens, J., Simintiras, C., Francis, J., Sun, J., & Fu, X. (2023). Progenitor cell isolation from mouse epididymal adipose tissue and sequencing library construction. STAR Protocols, 4 (4) https://doi.org/10.1016/j.xpro.2023.102703