Cryopreservation of leucocytes of channel catfish for subsequent cytogenetic analysis

Document Type

Article

Publication Date

1-1-1995

Abstract

Channel catfish leucocytes cryopreserved with glycerol or dimethyl sulphoxide (DMSO) had significantly higher (P<0.05) viability and recovery rates than did cells cryopreserved with methanol. After 7 days of frozen storage, a 24 to 27% reduction of viability was observed for cells cryopreserved with glycerol; a 25 to 43% reduction for cells frozen with DMSO, and a 67 to 100% reduction for cells frozen with methanol. The concentration of cryoprotectants affected the viability of cryopreserved cells significantly (p<0.05). The viability reduction was 36% for cells frozen with 5% of cryoprotectants, 30% for cells frozen with 10% of cryoprotectants, and 49% for cells frozen with 15% of cryoprotectants. The viability of cells frozen at the slower rate (‐2.7°C min−1) was significantly higher (p<0.05) than that of cells frozen at the faster rate (‐45°C min−1). Best results were obtained for cells cryopreserved with 10% of glycerol or DMSO and frozen at the slower rate. The chromosomes prepared from cells cryopreserved using this procedure were identical to those prepared from fresh cells, and to those reported in the literature for channel Catfish. Copyright © 1995, Wiley Blackwell. All rights reserved

Publication Source (Journal or Book title)

Journal of Fish Biology

First Page

1016

Last Page

1025

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