Document Type
Article
Publication Date
7-1-1998
Abstract
A replication banding procedure using fluorouracil (FU) and bromodeoxyuridine (BrdU) was developed for use with cultured leukocytes of channel catfish (Ictalurus punctatus). Analyzable banding patterns were produced on chromosomes stained with a fluorochrome plus Giemsa (FPG) method. The consistency of the chromosomal bands was evaluated by computer-assisted image analysis. Banding patterns of representative chromosomes including those bearing nucleolus organizer regions (NOR) were reproducible. A standard RBG-banded karyotype (R bands by BrdU and Giemsa) was established with idiograms of each chromosome. We also developed procedures to study sister- chromatid exchange (SCE) and sister-chromatid differentiation (SCD) in cultured leukocytes, without addition of mutagenic substances. The average occurrence of SCEs in the absence of mutagens was 3.0 ± 1.0 (n = 60) chromosomes per cell and was not significantly different among fish (P = .26).
Publication Source (Journal or Book title)
Journal of Heredity
First Page
348
Last Page
353
Recommended Citation
Zhang, Q., Wolters, W., & Tiersch, T. (1998). Replication banding and sister-chromatid exchange of chromosomes of channel catfish (Ictalurus punctatus). Journal of Heredity, 89 (4), 348-353. https://doi.org/10.1093/jhered/89.4.348