Document Type

Article

Publication Date

2-26-2004

Abstract

Sperm were collected in Florida from wild common snook. Centropomus undecimalis (Bloch), and were shipped to Louisiana State University for analysis and cryopreservation. Threshold activation of sperm (10% motility) occurred at 370 mOsmol kg-1, and complete activation occurred at 680 mOsmol kg-1. These values were significantly different. Sperm samples stored at 1°C in Hanks' balanced salt solution (HBSS) or in 0.6% NaCl solution at 200 mOsmol kg-1 retained motility for as long as 22 days. Mean motility remained above 50% for 9 days for sperm stored in HBSS and for 7 days for sperm stored in NaCl solution. Sperm exposed to 5% dimethyl acetamide (62 ± 10%; mean ± SD), 10% dimethyl sulphoxide (DMSO) (39 ± 16%), 5% glycerol (26 ± 5%) or 10% glycerol (6 ± 2%) for 30 min had significantly lower motility than did unexposed sperm (89 ± 9%). When used as a cryoprotectant, samples frozen with 5% or 10% DMSO or 5% methanol had significantly higher post-thaw motility than did samples frozen with other cryoprotectants. Sperm cryopreserved with 10% DMSO (38 ± 12%) had significantly higher post-thaw motility than did sperm cryopreserved with 15% DMSO (19 ± 10%) or 20% DMSO (4 ± 4%). There were no significant differences in hatch rates of eggs fertilized with fresh sperm (54 ± 29%) or cryopreserved sperm (41 ± 35%). Survival to first feeding was not different between fish produced with fresh sperm (37 ± 30%; range, 0-86%) or with thawed sperm (24 ± 29%; 0-77%). Transport of sperm to a cryopreservation laboratory and back to a hatchery for thawing and use enabled collaboration between groups with specific expertise and provides a model for the application of cryopreservation by transport of fresh and frozen samples.

Publication Source (Journal or Book title)

Aquaculture Research

First Page

278

Last Page

288

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