Outlook for development of high-throughput cryopreservation for small-bodied biomedical model fishes
Document Type
Conference Proceeding
Publication Date
1-1-2011
Abstract
With the development of genomic research technologies, comparative genome studies among vertebrate species are becoming commonplace for human biomedical research. Fish offer unlimited versatility for biomedical research. Extensive studies are done using these fish models, yielding tens of thousands of specific strains and lines, and the number is increasing every day. Thus, high-throughput sperm cryopreservation is urgently needed to preserve these genetic resources. Although high-throughput processing has been widely applied for sperm cryopreservation in livestock for decades, application in biomedical model fishes is still in the concept-development stage because of the limited sample volumes and the biological characteristics of fish sperm. High-throughput processing in livestock was developed based on advances made in the laboratory and was scaled up for increased processing speed, capability for mass production, and uniformity and quality assurance. Cryopreserved germplasm combined with high-throughput processing constitutes an independent industry encompassing animal breeding, preservation of genetic diversity, and medical research. Currently, there is no specifically engineered system available for high-throughput of cryopreserved germplasm for aquatic species. This review is to discuss the concepts and needs for high-throughput technology for model fishes, propose approaches for technical development, and overview future directions of this approach. © 2011 Elsevier Inc. All rights reserved.
Publication Source (Journal or Book title)
Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
First Page
76
Last Page
81
Recommended Citation
Tiersch, T., Yang, H., & Hu, E. (2011). Outlook for development of high-throughput cryopreservation for small-bodied biomedical model fishes. Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 154 (2), 76-81. https://doi.org/10.1016/j.cbpc.2011.03.004