Document Type
Article
Publication Date
3-1-2017
Abstract
If biosurfactants are to achieve their promise in environmental oil-spill remediation, their toxicity to marine life must be assessed. A killifish larvae assay is commonly used as a measure of toxicity but is difficult and nonlinear in response. Red blood cell (RBC) hemolysis has also been the basis for assays of some surfactant levels. Here we present a modified sheep RBC suspension assay and compare its response to that of the fish assay for surfactin and its genetically modified variant fatty-acyl-glutamate (FA-Glu). This is the first report of hemolytic activity as a property of FA-Glu. The method's potential for screening for toxicity against marine organisms is demonstrated. © 2016 American Institute of Chemical Engineers Environ Prog, 36: 505–510, 2017.
Publication Source (Journal or Book title)
Environmental Progress and Sustainable Energy
First Page
505
Last Page
510
Recommended Citation
Colonna, W., Martı, M., Nyman, J., Green, C., & Glatz, C. (2017). Hemolysis as a rapid screening technique for assessing the toxicity of native surfactin and a genetically engineered derivative. Environmental Progress and Sustainable Energy, 36 (2), 505-510. https://doi.org/10.1002/ep.12444