Document Type
Article
Publication Date
8-1-2010
Abstract
Cryptotanshinone (CPT), a natural compound isolated from the plant Salvia miltiorrhiza Bunge, is a potential anticancer agent. However, little is known about its anticancer mechanism. Here, we show that CPT inhibited cancer cell proliferation by arresting cells in G1-G0 phase of the cell cycle. This is associated with the inhibition of cyclin D1 expression and retinoblastoma (Rb) protein phosphorylation. Furthermore, we found that CPT inhibited the signaling pathway of the mammalian target of rapamycin (mTOR), a central regulator of cell proliferation. This is evidenced by the findings that CPT inhibited type I insulin-like growth factor I- or 10% fetal bovine serum - stimulated phosphorylation of mTOR, p70 S6 kinase 1, and eukaryotic initiation factor 4E binding protein 1 in a concentration- and time-dependent manner. Expression of constitutively active mTOR conferred resistance to CPT inhibition of cyclin D1 expression and Rb phosphorylation, as well as cell growth. The results suggest that CPT is a novel antiproliferative agent. ©2010 AACR.
Publication Source (Journal or Book title)
Cancer Prevention Research
First Page
1015
Last Page
1025
Recommended Citation
Chen, W., Luo, Y., Liu, L., Zhou, H., Xu, B., Han, X., Shen, T., Liu, Z., Lu, Y., & Huang, S. (2010). Cryptotanshinone inhibits cancer cell proliferation by suppressing mammalian target of rapamycin - Mediated cyclin D1 expression and Rb phosphorylation. Cancer Prevention Research, 3 (8), 1015-1025. https://doi.org/10.1158/1940-6207.CAPR-10-0020