HPLC-MS determination and pharmacokinetic study of 7, 8-dihydroxyflavone, a naturally-occurring neuroprotective agent after a single oral administration in rats
Document Type
Article
Publication Date
1-1-2018
Abstract
Background: 7, 8-dihydroxyflavone (7, 8-DHF) has been discovered to be a TrkB receptor-specific agonist and demonstrated remarkable therapeutic efficacy in vivo. These promising findings prompted the current pharmacokinetic study, in which we first established a practical LC-MS quantitative method for 7, 8-DHF then applied it in a pilot pharmacokinetic study in rats. Methods: A simple HPLC-MS system was employed for the determination of 7, 8-DHF in rat plasma after the plasma samples were extracted with ethyl acetate by vortex-mixing. The method was thoroughly validated regarding accuracy, precision, sensitivity, linearity, selectivity, recovery, and stability. Results: This method exhibited linearity within the concentration range of 9.024 (LLOQ) and 1128 ng/mL. The accuracy ranged from 87.9 to 105.6% of the actual values. The intra-and inter-day precisions ranged from 1.7 to 5.1% and from 2.2 to 4.4% (RSD, %). In the pharmacokinetic study, 7, 8-DHF was detectable in rat plasma for up to 24 h, with a terminal half-life of 22.8 h. Conclusion: Overall, the results showed that this method was fully validated and successfully applied to a pilot pharmacokinetic study, which will facilitate the determination of the pharmacokinetic profile of 7, 8-DHF in future animal studies.
Publication Source (Journal or Book title)
Current Pharmaceutical Analysis
First Page
343
Last Page
348
Recommended Citation
Chen, J., Fu, X., Dong, Y., Liu, M., Zhang, J., & Liu, Z. (2018). HPLC-MS determination and pharmacokinetic study of 7, 8-dihydroxyflavone, a naturally-occurring neuroprotective agent after a single oral administration in rats. Current Pharmaceutical Analysis, 14 (4), 343-348. https://doi.org/10.2174/1573412913666170410132032
