Chicken erythrocytes as an internal reference for analysis of dna content by flow cytometry in grass carp

Document Type

Article

Publication Date

1-1-1989

Abstract

We estimated the nuclear DNA content of unfixed erythrocytes of the grass carp Ctenopharyngodon idella by fluorescence flow cytometry using propidium iodide as a fluorochrome. Frozen erythrocytes from the domestic chicken Gallus gallus were thawed and used as an internal reference for simultaneous analysis with grass carp samples. The nuclear DNA content of erythrocytes from diploid grass carp (mean ± SD) was 2.00 ± 0.01 pg/cell. whereas triploids possessed 2.97 ± 0.03 pg/cell. Variations in the nuclear DNA content of different chickens were standardized in relation to the DNA content of fresh human leukocytes (7.00 pg/cell). The value for DNA content of the chicken erythrocytes (around 2.5 pg/cell) occupied a position intermediate between the two grass carp values, and thus provided a clear reference for discrimination between diploids and triploids. © 1989 by the American fisheries society.

Publication Source (Journal or Book title)

Transactions of the American Fisheries Society

First Page

713

Last Page

717

This document is currently not available here.

Share

COinS