Studies in macaques on cross-clade T cell responses elicited by a DNA/MVA AIDS vaccine, better conservation of CD8 than CD4 T cell responses
Document Type
Article
Publication Date
2-1-2005
Abstract
One of the unknowns faced by an HIV/AIDS vaccine is the ability of a single clade vaccine to protect against the multiple genetic subtypes and recombinant forms of HIV-1 present in the current pandemic. Here, we use a macaque model to investigate the ability of our clade B vaccine that consists of DNA priming and modified vaccinia Ankara (MVA) virus boosting to elicit T cell responses that recognize an A/G recombinant of HIV-1. To test for cross-reactive T cells, intracellular cytokine staining was conducted using five pools of Gag and six pools of Env peptides representing B or A/G sequences. Studies using the peptide pools revealed essentially complete conservation of the CD8 response but only ∼50% conservation of the CD4 response. Thus, the ability of an HIV vaccine for one clade to protect against other clades may be more limited by the ability to provide CD4 T cell help than the ability to elicit CD8 effector functions.
Publication Source (Journal or Book title)
AIDS Research and Human Retroviruses
First Page
140
Last Page
144
Recommended Citation
Smith, J., Amara, R., Wyatt, L., Ellenberger, D., Li, B., Herndon, J., Patel, M., Sharma, S., Chennareddi, L., Butera, S., McNicholl, J., McClure, H., Moss, B., & Robinson, H. (2005). Studies in macaques on cross-clade T cell responses elicited by a DNA/MVA AIDS vaccine, better conservation of CD8 than CD4 T cell responses. AIDS Research and Human Retroviruses, 21 (2), 140-144. https://doi.org/10.1089/aid.2005.21.140