Microarrays of DNA and protein integrated on microfluidic chip

Authors

Zhi Xiang Zhang, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Zheng Shen, Dalian Institute of Chemical Physics Chinese Academy of Sciences
Hui Zhao, Shanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences
Bin Li, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Shi Ping Song, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Jun Hu, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Bing Cheng Lin, Dalian Institute of Chemical Physics Chinese Academy of Sciences
Min Qian Li, Shanghai Institute of Applied Physics, Chinese Academy of Sciences

Document Type

Article

Publication Date

12-1-2005

Abstract

To combine the advantages of high-throughput microarray and microfluidic chip technology, microarray was fabricated in channels. DNA and protein were arrayed on quartz slide, which was reversibly sealed by poly(dimethylsiloxane) (PDMS) slab containing pattern of channels. The hybridization carried out in channels indicated that target DNA and proteins were bound to their complementary DNA and protein probes specifically. In this method, PDMS conjugated with polylysine was contributed to imparting the wettability and decreasing unspecific adsorption of biomolecules. Our data demonstrated that the integrated chip speeded up the detection compared to microarrays, extended the parallel channels with serial properties and enhanced the throughput of probes and targets.

Publication Source (Journal or Book title)

Acta Chimica Sinica

First Page

1743

Last Page

1746

Recommended Citation

Zhang, Z., Shen, Z., Zhao, H., Li, B., Song, S., Hu, J., Lin, B., & Li, M. (2005). Microarrays of DNA and protein integrated on microfluidic chip. Acta Chimica Sinica, 63 (18), 1743-1746. Retrieved from https://repository.lsu.edu/ag_exst_pubs/918