Nano-manipulation of single DNA molecules based on atomic force microscopy

Document Type

Conference Proceeding

Publication Date

12-1-2005

Abstract

Nano-manipulation of single atoms and molecules is a critical technique in nanoscience and nanotechnology. This paper will focus on the recent development of the manipulation of single DNA molecules based on atomic force microscopy (AFM) in our laboratory. Precise manipulation has been realized including varied manipulating modes such as "cutting", "pushing", "folding", "kneading", "picking up", "dipping", etc. The cutting accuracy is dominated by the size of the AFM tip, which is usually 10nm or less. Single DNA fragments can be cut and picked up and then amplified by single molecule PCR. Thus positioning isolation and sequencing can be performed. © 2005 IEEE.

Publication Source (Journal or Book title)

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

First Page

7478

Last Page

7481

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