Biotransformation of dichotomin by Pectinex BE XXL

Document Type

Article

Publication Date

1-1-2009

Abstract

AIM: To study of structural modification in steroidal saponin by biotransformation technology. METHODS: Structural modification was carried out of glycosyl residues of dichotomin at C-3 position by Pectinex BE XXL (PBX). Cytotoxicity against HL-60 cells of all products was investigated. RESULTS: The PBX could hydrolyze the glycosyl residues of dichotomin one-by-one to produce nine transformed compounds. The transformed product (mixture) and four secondary spirostanosides among them exhibited cytotoxicity. Compounds P5, P6 and P7 with α-1, 2 end-rhamnosyl residues showed a potential cytotoxic effect on HL-60 cells; P8 with α-1, 4 end-rhamnosyl residue had a weak effect on HL-60 cells. Dichotomin, secondary furostanosides and aglycone were not cytotoxic. CONCLUSIONS: PBX has various hydrolyzation to the glycosidic bonds of dichotomin. Nine obtained transformed compounds widen steroidal saponin library. Meanwhile, screening activity affords data for further pharmacological study. Copyright©2009, China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

Publication Source (Journal or Book title)

Chinese Journal of Natural Medicines

First Page

381

Last Page

389

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