Enzymatic digestion of single DNA molecules anchored on nanogold-modified surfaces

Authors

Junhong Lü, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Ming Ye, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Na Duan, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Bin Li, Shanghai Institute of Applied Physics, Chinese Academy of Sciences

Document Type

Article

Publication Date

6-5-2009

Abstract

To study enzyme-DNA interactions at single molecular level, both the attachment points and the immediate surroundings of surfaces must be carefully considered such that they do not compromise the structural information and biological properties of the sample under investigation. The present work demonstrates the feasibility of enzymatic digestion of single DNA molecules attached to nanoparticle-modified surfaces. With Nanogold linking DNA to the mica surface by electrostatic interactions, advantageous conditions with fewer effects on the length and topography of DNA are obtained, and an appropriate environment for the activities of DNA is created. We demonstrate that by using Dip-Pen Nanolithography, individual DNA molecules attached to modified mica surfaces can be efficiently digested by DNase I. © to the authors 2009.

Publication Source (Journal or Book title)

Nanoscale Research Letters

First Page

1029

Last Page

1034

Recommended Citation

Lü, J., Ye, M., Duan, N., & Li, B. (2009). Enzymatic digestion of single DNA molecules anchored on nanogold-modified surfaces. Nanoscale Research Letters, 4 (9), 1029-1034. https://doi.org/10.1007/s11671-009-9350-6