Document Type
Article
Publication Date
6-1-2011
Abstract
The study shows for the first time the presence of the Klebsiella oxytoca strain fp10 coproducing plasmid-mediated KPC-2 and IMP-8 carbapenemases. The strain was obtained from the fecal sample of an inpatient and showed high-level resistance to imipenem and ertapenem (MICs > 32 μg/ml). Conjugation experiments demonstrated the transferability of the carbapenem-resistant determinants. The results of plasmid analysis and Southern hybridization revealed that the bla KPC-2 gene was located on transferable plasmid pFP10-1 (∼54 kb), whereas the bla IMP-8 gene was on transferable plasmid pFP10-2 (∼180 kb). Analysis of the genetic environment of these two genes has demonstrated that ISKpn6 and ISKpn8 are involved in the spread of the bla KPC-2 gene, while the transposable elements IS26, intI1, and tniC might contribute to the dissemination of the bla IMP-8 gene. The chimera of several transposon-associated elements indicated a novel genetic environment of IMP-type metallo-β-lactamase gene in Enterobacteriaceae from China. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
Publication Source (Journal or Book title)
Antimicrobial Agents and Chemotherapy
First Page
2937
Last Page
2941
Recommended Citation
Li, B., Sun, J., Liu, Q., Han, L., Huang, X., & Ni, Y. (2011). First report of Klebsiella oxytoca strain coproducing KPC-2 and IMP-8 carbapenemases. Antimicrobial Agents and Chemotherapy, 55 (6), 2937-2941. https://doi.org/10.1128/AAC.01670-10
