Facilitating the pickup of individual DNA molecules by AFM nanomanipulation with tips mechanically worn on bare mica
Document Type
Article
Publication Date
1-1-2012
Abstract
The tip is one of the critical factors to improve the efficiency in picking up individual DNA molecules from solid substrates based on atomic force microscope (AFM) nanomanipulation. We found that wearing AFM tips on certain solid substrates in advance to nanomanipulation operation would largely improve the pickup efficiency, which was ascribed to the increasing affinity of the tip to the DNA molecules along with the increase of the tip radius after wearing. It was demonstrated that bare mica was superior to APTES-modified mica to keep the tip clean while wearing, which was crucial for DNA pickup during AFM nanomanipulation. © 2011 Wiley Periodicals, Inc.
Publication Source (Journal or Book title)
Microscopy Research and Technique
First Page
638
Last Page
642
Recommended Citation
Duan, N., Long, F., Wang, X., Li, B., Hu, J., & Zhang, Y. (2012). Facilitating the pickup of individual DNA molecules by AFM nanomanipulation with tips mechanically worn on bare mica. Microscopy Research and Technique, 75 (5), 638-642. https://doi.org/10.1002/jemt.21104