Characterization and production of tetraspore-derived seedlings of Grateloupia lanceolata (Halymeniales, Rhodophyta) in northeastern China
Document Type
Article
Publication Date
5-1-2012
Abstract
In this study, Grateloupia lanceolata, newly recorded from northeastern China, was classified on the basis of morphology and rbcL sequence analysis and also raised in a laboratory to evaluate the feasibility of producing tetraspore-derived seedlings. Analysis of the rbcL sequence showed the samples to be monophyletic with G. lanceolata and different from any other species of the genus. Analysis of variance indicated that time has a significant (P< 0.01) effect on both tetraspore output and the percentage of total tetraspores released; those released in the first 2 d made up more than 72% of the total sporulation output; whereas, those released in the last 2 d contributed only 2.52%. In culture, tetraspores released from the unilocular sporangia developed into small discs in one week, and 87% ±6 5.7% of the tetraspores that settled developed into sporelings after 9 wk of culture. Tetraspore culture of G. lanceolata in tanks and in the open sea proved to be a feasible method of generating large numbers of individual gametophytes economically.
Publication Source (Journal or Book title)
Phycologia
First Page
330
Last Page
339
Recommended Citation
Ye, N., Zhang, X., Wang, H., Lian, W., Mao, Y., Xu, D., Li, B., Liang, C., Zou, J., Zhu, B., Jiang, Z., Gao, Z., & Li, D. (2012). Characterization and production of tetraspore-derived seedlings of Grateloupia lanceolata (Halymeniales, Rhodophyta) in northeastern China. Phycologia, 51 (3), 330-339. https://doi.org/10.2216/11-03.1