Fine structure of DNA bundles: Atomic force microscopy study
Document Type
Article
Publication Date
12-1-2001
Abstract
Direct observations of fine structure of DNA bundles were presented in this paper. These DNA bundles existed originally in λ-DNA solution with a high concentration of 480 ng/μl. When this solution was diluted, DNA bundles finally split into individual DNA molecules. DNA bundles were stretched on mica treated with 3-aminopropyl triethoxysilane (APS) and observed by an atomic force microscope (AFM). A method was developed to avoid the possible artifacts during the drying process in sample preparation. It was found that in a bundle composed of two individual DNA molecules, these two molecules wound around each other in a helical path.
Publication Source (Journal or Book title)
Japanese Journal of Applied Physics, Part 2: Letters
Recommended Citation
Li, G., Zhang, Y., Li, B., Hu, J., & Li, M. (2001). Fine structure of DNA bundles: Atomic force microscopy study. Japanese Journal of Applied Physics, Part 2: Letters, 40 (12 A) https://doi.org/10.1143/jjap.40.l1330
