Determination of Bioavailable Cadmium in Environmental Samples by IP-RP-HPLC Coupled with On-line Fluorescence Assay

Document Type

Article

Publication Date

9-1-2002

Abstract

A novel reserved-phase ion-pair liquid chromatographic method using hexadecyltrimethylammonium bromide (HTMAB) as an ion-pair reagent for separating the complexes of metal and 8-hydroxyquinoline-5-sulphonic acid(HQS) coupled with on-line fluorescence detection has been developed. The chromatographic behavior of metal-HQS complexes in the proposed system was systematically investigated. When the C18 column modified by HTMAB and the suitable mobile phase coupled with fluorescence detection(λex=388 nm; λem=518 nm) were employed, the detection limit of cadmium is 7. 72 ng/ mL with an injection volume of 20 μL, and the linear range of calibration curve for cadmium is 50-800 ng/mL. It was successfully applied to determination of bio-available cadmium in polluted soils of China. Moreover, the possible species of Cd-HQS on the column has been investigated by log-log plot analysis.

Publication Source (Journal or Book title)

Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities

First Page

1694

This document is currently not available here.

Share

COinS