Cloning and expression analysis of violaxanthin De-expoxidase (VDE) cDNA in wheat

Document Type

Article

Publication Date

8-1-2003

Abstract

Violaxanthin de-epoxidase (VDE) is the key enzyme in the xanthophyll cycle and protects plant photosynthetic apparatus from the damage of excessive light. A wheat (Triticum aestivum L. cv. Xiaoyan 54) VDE cDNA was obtained using RT-PCR method. Its deduced protein sequence shares high identity with that of Arabidopsis and rice. Southern blot revealed that there are three copies of VDE gene per haploid genome of wheat. VDE transcript levels were higher in green leaf than in root, seed and etiolated leaf. Northern blotting analysis indicated that VDE mRNA level is induced during greening process of etiolated wheat seedling and increased by intense light illumination.

Publication Source (Journal or Book title)

Acta Botanica Sinica

First Page

981

Last Page

985

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