Real time observation of the photocleavage of single DNA molecules
Document Type
Article
Publication Date
12-1-2003
Abstract
A method for real time observation of photocleavage of stretched λDNA single molecular level by a fluorescent microscope coupled with CCD is developed. DNA molecules stained with YOYO-1 are stretched by the molecular combing technique and fixed on a modified slide. Then the process of photocleavage and relaxation of DNA under radiation of blue light is observed. We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleavage DNA molecules. The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity, cancer research as well as the interaction between DNA and dyes.
Publication Source (Journal or Book title)
Chinese Science Bulletin
First Page
673
Last Page
675
Recommended Citation
Li, B., Hu, J., Wang, Y., Wu, S., Huang, Y., & Li, M. (2003). Real time observation of the photocleavage of single DNA molecules. Chinese Science Bulletin, 48 (7), 673-675. https://doi.org/10.1360/03tb9144
