Directional solidification stage with dynamically variable speeds: Assessment of cell viability after interrupted cooling

Document Type

Conference Proceeding

Publication Date

12-1-2011

Abstract

A two-step directional freezing method was utilized to assess the membrane integrity in frozen/thawed adult stem cells (ASCs). Using a custom built directional solidification stage (DSS) we determined that cells cooled at two different rates (i.e., a slower rate between room temperature, 27 °C and -10°C and a higher rate between -10°C and -60°C) experienced significantly higher loss of cell viability when compared with those cooled at a single and uniform slower cooling rate between 27°C and -60°C. Specifically, the cells cooled using the two-step freezing method exhibited a post-thaw cell viability of ∼17% while those that were cooled using the traditional method exhibited a cell viability of 49%. We also found that varying the cooling rate between 27°C and -10°C did not significantly affect the post-thaw viability of ASCs, suggesting that they are not susceptible to supra-zero cooling injury. Copyright © 2011 by ASME.

Publication Source (Journal or Book title)

ASME 2011 Summer Bioengineering Conference, SBC 2011

First Page

923

Last Page

924

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