Uncoil long DNA molecules by nanoposts array
Document Type
Conference Proceeding
Publication Date
8-17-2012
Abstract
A consistent and reproducible degree of stretching of DNA is important for single DNA molecule analysis such as DNA mapping and fast sequencing. Micro/nanopost or posts arrays are known to be able to uncoil genomic scale DNAs upon collision on the posts. Here, we built periodical hexagon nanoposts array embedded in a polymeric micro/nanofluidic device at low costs by a novel NanoImprinting process. The electrophoresis of λ-DNA and T4 DNA were studied in the posts array. A periodical stretching/recoiling motion was observed for both DNAs. Experiments showed a stretching to over 90% of its contour length was highly reproducible for both λ-DNA and T4 DNA. This non-gel electrophoresis of DNA also can be useful for the study of fundamental of DNA separation.
Publication Source (Journal or Book title)
Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012
First Page
380
Last Page
383
Recommended Citation
Wu, J., Shao, G., Choi, J., Uba, F., Soper, S., & Park, S. (2012). Uncoil long DNA molecules by nanoposts array. Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012, 380-383. Retrieved from https://repository.lsu.edu/mechanical_engineering_pubs/1930