Integrated thermoplastic nanofluidic device for detection of chemotherapeutically induced damage in genomic DNA

Authors

K. M. Weerakoon-Ratnayake, The University of North Carolina at Chapel Hill
V. Singh, The University of North Carolina at Chapel Hill
F. I. Uba, The University of North Carolina at Chapel Hill
R. M. Schotzinger, The University of North Carolina at Chapel Hill
J. Choi, Louisiana State University
Z. Jia, Louisiana State University
P. D. Chastain, The University of North Carolina at Chapel Hill
D. G. Kaufman, The University of North Carolina at Chapel Hill
S. Park, Louisiana State University
S. A. Soper, The University of North Carolina at Chapel Hill

Document Type

Conference Proceeding

Publication Date

1-1-2016

Abstract

We are developing an integrated nanofluidic device capable of identifying DNA damage (abasic sites) induced by chemotherapy for genomic DNA extracted from Circulating Tumor Cells (CTCs) of cancer patients. The biofluidic processor will enable isolating CTC's directly from patient's peripheral blood, extract/enrich the genomic DNA from cell lysates and quantify the abasic (AP) sites using optical and electrical detection modalities in near real time.

Publication Source (Journal or Book title)

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

First Page

1099

Last Page

1101

Recommended Citation

Weerakoon-Ratnayake, K., Singh, V., Uba, F., Schotzinger, R., Choi, J., Jia, Z., Chastain, P., Kaufman, D., Park, S., & Soper, S. (2016). Integrated thermoplastic nanofluidic device for detection of chemotherapeutically induced damage in genomic DNA. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 1099-1101. Retrieved from https://repository.lsu.edu/mechanical_engineering_pubs/1917