Polymer nanosensors using electrophoretic identification of nucleotides for single-molecule sequencing

Document Type

Conference Proceeding

Publication Date

1-1-2018

Abstract

We are generating a single-molecule platform (X-EPM, Figure 1) that can acquire sequencing information with high accuracy (>95%). The platform reads the identity of single mononucleotides from their molecular-dependent electrophoretic mobility. Two-dimensional (2D) nanochannels (50 nm in width and depth; ∼10 μm in length) are fabricated in a thermoplastic via nanoimprint lithography (NIL) to deduce the mobility. The mononucleotides are generated from a DNA/RNA molecule using a processive exonuclease, which is covalently anchored to a solid support that sequentially feeds mononucleotides into the 2D nanochannel. The mobility is read by measuring current transients from <10 nm (diameter) in-plane pores.

Publication Source (Journal or Book title)

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

First Page

2455

Last Page

2456

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