Semester of Graduation

Summer 2022


Master of Science (MS)


Veterinary Clinical Sciences

Document Type



Topical products are essential treatments for canine superficial bacterial folliculitis. However, studies for commercial products typically evaluate antibacterial effects on hairs rather than the skin surface. In addition, the interference from the hair length has not been evaluated in these studies. Therefore, the study objective for this study was to evaluate the residual antibacterial effects of five mousse products, (1) 2% chlorhexidine and 2% miconazole, (2) 0.05% phytosphingosine, (3) 2% salicylic acid and 10% ethyl lactate, (4) 3% chlorhexidine and 0.5% climbazole, and (5) 2% chlorhexidine and 1% ketoconazole on both the skin surface and hairs in both short- and long-haired dogs. Products were applied once on 15 short-haired and eight long-haired dogs without dermatologic disease and which had not been treated with topical or systemic antimicrobial products within the previous four weeks. Skin surface swabs and hairs were collected from the trunk before treatment, and at one hour and at two, four, seven, 10, and 14 days post-treatment. Samples were placed on Mueller–Hinton plates pre-streaked with non-antibiotic resistant Staphylococcus pseudintermedius. Each dog’s samples per time point were placed on the same plate. Inhibition zones were measured after 24 h of incubation. No inhibition was seen with pre-treatment samples or with products 2 and 3. In short-haired dogs, both skin swabs and hairs from areas treated with mousses 1, 4, and 5 produced post-treatment inhibition until Day 14 (p < 0.001). In long-haired dogs, treated hairs demonstrated inhibition until Day 14 with mousses 4 and 5 (p < 0.001) but only until Day 10 with mousse 1 (p < 0.001). However, skin swabs from mousse 1 only produced inhibition until Day 4 in long-haired dogs (p < 0.001). In conclusion, evaluation of hairs may overestimate bacterial inhibition on the skin surface, especially in long-haired dogs.



Committee Chair

Pucheu-Haston, Cherie