Master of Science (MS)


School of Nutrition and Food Sciences

Document Type



The present study was conducted to determine the influence of an ozonation process on lutein and protein in clean and contaminated corns. This study aimed (1) to determine the levels of antimutagenic compounds to aflatoxin in corn before and after ozonation; (2) to verify the antimutagenic potential of these compounds against aflatoxin using the Ames test; and (3) to evaluate proteins in the corn before and after ozonation. HPLC was used to separate and identify the lutein in corn. Nitrogen analysis and SDS-PAGE were used to analyze protein content and molecular weights in corn. Clean ozone-treated corn had a total lutein content of 28.36 ug/g, which was higher than that of 22.75 ug/g in the untreated clean corn. However, the results of contaminated corn were contrary to clean corn. Lutein content of 11.69 ug/g in the ozone-treated contaminated corn was lower than that of 16.42 ug/g in the untreated contaminated corn. Through the nitrogen analysis, protein content was determined. In both corn samples, the protein content of ozone-treated corn was lower than that of untreated corn, indicating that protein could be destroyed by the ozonation process. The Ames test using the Salmonella plate incorporation method was used to examine the effect of lutein extracts from corn on aflatoxin B1 mutagenicity, using TA100 tester strain. Lutein extracts alone showed no mutagenic potential against Samonella typhimurium tester strains TA 100. The dose of 500 ng AFB1/g was chosen for the antimutagenicity studies. Pure lutein and lutein extracts from corn inhibited the mutagenicity of AFB1 in a dose-response manner. Lutein extracts were more efficient at inhibiting AFB1 mutagenicity than lutein standard. Lutein extracts from different corn samples had similar antimutagenic potential against AFB1, so the ozone treatment could not change antimutagenic potential of lutein extracts. Ozonation releases lutein from clean corn, while in the contaminated corn, ozonation destroys some of lutein in the corn. However, ozonation dose not change the antimutagenic potential of lutein against AFB1. Protein can be destroyed by ozone, which influences the nutritious value of corn.



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Committee Chair

Joan M. King



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Life Sciences Commons