Date of Award


Document Type


Degree Name

Doctor of Philosophy (PhD)


Inulin, a polyfructan, is found as the reserve carbohydrate in the roots and tubers of various plants. The (beta)-fructofuranosidase (inulase) from the yeast Kluyveromyces fragilis is of interest because of its industrial potential in fructose syrup and alcohol production from inulin containing plants. The enzyme was purified and characterized with respect to its physical and kinetic parameters. The relationship between the enzyme and the yeast cell wall was also investigated. It was found that the inulase of Kluyveromyces fragilis could be immobilized in the yeast cells by glutaraldehyde treatment. Although the exact nature of the immobilization is not fully understood the kinetic parameters of the immobilized enzyme are similar to those of the soluble enzyme. The cells are resistant to physical and enzymatic destruction and warrant further investigation as to their use in an enzymatic reactor for the production of high fructose syrup from inulin.