Effects of nutritional conditions on the stable carbon and nitrogen isotope of microalgae

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With five microalgae (dinoflagellates, diatoms, green algae species) as test materials, the effect of different nutrient conditions had been explored on the stable carbon and nitrogen isotopic throughout the growth phases. The result showed that among different classes of microalgae the δ13C and δ15N at the exponential phase were lower than those at the stable phase, indicating no correlation with growth velosity (P > 0.05). Bacillariophyceae presented a higher δ13C than dinophyceae and chlorophyceae, while the δ13C of dinophyceae was nearly identical to that of chlorophyceae. In terms of δ15N, the dinophyceae falls behind both bacillariophyceae and chlorophyceae, with bacillariophyceae approximating to chlorophyceae. In addition, significant δ15N differences have been discovered among Prorocentrum micans, Alexandrium tamarense and Amphidinium carterae Hulburt. In this study, all five classes were grown under three distinctive cultures-nutrient sufficiency, nitrogen deficiency and phosphorus deficiency; either nitrogen or phosphorus limitation contributed to a more positive δ13C and δ15N, yet less obvious effect was observed in phosphorus-deficiency culture than in nitrogen-deficiency condition.

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Zhongguo Huanjing Kexue/China Environmental Science

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