Glutathione S-transferase in the house fly: Biochemical and genetic changes associated with induction and insecticide resistance
Document Type
Article
Publication Date
1-1-1984
Abstract
Kinetic parameters were measured for glutathione S-transferase, an enzyme important in metabolic resistance to insecticides, in one susceptible and two insecticide-resistant strains of the house fly (Musca domestica L.), and in untreated and chemically induced flies. Both resistant strains differed from the susceptible strain in apparent Km values for the enzyme, while only one differed in apparent Vmax. Two of the strains were inducible with phenobarbital; the third with 3-methylcholanthrene. Kinetic analysis indicated enzyme induction was associated with changes in Km rather than Vmax, and genetic experiments showed that most variation relating to Km and Vmax was controlled by chromosome II. Based on these results, both metabolic resistance and induction of enzyme activity were associated primarily with the production of different forms of glutathione S-transferase rather than more of the enzyme present in susceptible flies. © 1984.
Publication Source (Journal or Book title)
Pesticide Biochemistry and Physiology
First Page
203
Last Page
208
Recommended Citation
Ottea, J., & Plapp, F. (1984). Glutathione S-transferase in the house fly: Biochemical and genetic changes associated with induction and insecticide resistance. Pesticide Biochemistry and Physiology, 22 (2), 203-208. https://doi.org/10.1016/0048-3575(84)90091-9