Mass spectrometry-based proteomics for structure and function determination of alligator leukocyte proteins

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Mass spectrometry-based proteomics can be used to investigate the properties of alligator leukocytes and better understand the alligator innate immune system. Proteomics is critical to understanding complex biological systems through the elucidation of protein expression, function, modifications, and interactions. Separation techniques, including liquid chromatography, gel electrophoresis, and ion mobility as well as mass spectrometry tools such soft ionization and high resolution mass separation are available for proteomic applications. With organisms such as reptiles, for which there is limited genomic and proteomic data, a de novo sequencing approach can be implemented. In this chapter, we describe the use of mass spectrometry-based proteomics to determine peptide sequences from alligator leukocyte proteins and identify proteins based on sequence homology. A description of the instruments and methods used for analyzing peptides and proteins isolated from alligator leukocytes is provided. © 2012 by Nova Science Publishers, Inc. All Rights Reserved.

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Leukocytes: Biology, Classification and Role in Disease

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