Three-dimensional biologically-active nanostructured architectures in polymer-based microfluidic devices for biomarker detection

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Conference Proceeding

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We report a novel three-dimensional (3D) DNA analysis device consisting of ultrahigh-aspect-ratio polymer nanopillars prepared by a templating polymerization patterntransfer method followed by a direct-write photochemical patterning approach to produce functional scaffolds for the immobilization of molecular recognition probes to nanopillars. These nanopillars can be integrated into microfluidic channels for producing high-surface-area supports for these probes to increase assay sensitivity and dynamic range, as well as rapid response times due to microfluidic addressing of the array. Anodic aluminum oxide (AAO) was used as the template for the nanostructures as well as the molding tool for the microfluidic network. The AAO template consisted of nanopores with a close-packed hexagonal geometry. The device demonstrated highspeed hybridization kinetics with detection limits on the order of femtomoles. © 2006 Society for Chemistry and Micro-Nano Systems.

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Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences

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