Multi-analyte imprinting capability of OMNiMIPs versus traditional molecularly imprinted polymers

Document Type


Publication Date



One monomer molecylarly imprinted polymers (OMNiMlPs) have enhanced binding and selectivity properties versus traditionally formulated ethylene glycol dimethacrylate (EGDMA)/methacryIic acid (MAA) imprinted polymers. Further comparison was investigated toward multi-analyte imprinting capability of these two imprinted materials. Two templates, (R)-(+)-1,1'-bi-2-naphthol and BOC-L-tyrosine were simultaneously imprinted in the polymers, and the enantioselectivity compared to polymers imprinted with one template at a time. The simultaneously imprinted OMNiMIP exhibited only 63 and 21.1% loss in enantioselectivity for (R)-(+)-1,1'-bi-2-naphthol and BOC-L-tyrosine respectively, versus the singly imprinted OMNiMlPs. For the EGDMA/MAA imprinted polymer, enantioselectivity was only found for (R)-(+)-1,1'-bi-2-naphthol, with 59.1% loss in enantioselectivity found for the multiple-template imprinted polymer wersus the (R)-(+)-1,1'-bi-2-naphthol singly imprinted polymer. It was also shown that imprinting two templates simultaneously leads to better enantioselective performance than mixing the particles of singly imprinted polymers. For example, the enantioselectivity of the R enantiomer of 1,1 '-bi-2-binapthol on the simultaneously imprinted OMNiMIP gawe a separation factor (α) value of 4.4, while the mixed-particle column gave an α walue of 2.6. In addition, it was found that mixing an imprinted polymer with a non-imprinted polymer resulted in complete loss of chromatographic enantioselectiwity in all cases (except the one that still showed sewere loss of selectivity). Collectively, the results illustrate that imprinting mixtures of templates simultaneously is the method of choice, especially for OMNiMlPs, for producing multi-analyte molecular recognition in imprinted polymers. Copyright © 2008 John Wiley & Sons, Ltd.

Publication Source (Journal or Book title)

Journal of Molecular Recognition

First Page


Last Page


This document is currently not available here.