Stretching DNA molecules with a centrifugal method and imaging with atomic force microscopy

Authors

Y. Zhang, Chinese Academy of Sciences
Q. Xiong, Chinese Academy of Sciences
B. Li, Chinese Academy of Sciences
S. Lou, Chinese Academy of Sciences
Z. Ouyang, Chinese Academy of Sciences
J. Hu, Chinese Academy of Sciences
M. Li, Chinese Academy of Sciences

Document Type

Article

Publication Date

12-1-2000

Abstract

A convenient method has been developed for stretching DNA molecules on chemical-modified mica surfaces by using conventional centrifugal methods. Atomic force microscopy images of the stretched DNA were obtained, which demonstrated that this approach was able to stretch not only long DNA strands such as λDNA but also short DNA with length of less than 300 nm. It was found that the pH of the DNA solution affected DNA-stretching efficiency. The mechanism has been discussed.

Publication Source (Journal or Book title)

Probe Microscopy

First Page

31

Last Page

36

Recommended Citation

Zhang, Y., Xiong, Q., Li, B., Lou, S., Ouyang, Z., Hu, J., & Li, M. (2000). Stretching DNA molecules with a centrifugal method and imaging with atomic force microscopy. Probe Microscopy, 2 (1), 31-36. Retrieved from https://repository.lsu.edu/ag_exst_pubs/1083