Identification and functional analysis of Arabidopsis proteins that interact with resistance gene product RPS2 in yeast
Document Type
Article
Publication Date
11-1-2004
Abstract
A yeast-two-hybrid screen was used to investigate the interaction controlled by resistance gene RPS2 of Arabidopsis thaliana and avirulence gene avrRpt2 of Pseudomonas syringae. Twelve Arabidopsis proteins that physically interact with RPS2 in yeast were identified. Four of these were studied further including a calmodulin, a putative ankyrin/tetratricopeptide repeat-containing protein, a protein with a nuclear localization domain, and a protein of unknown function. In yeast, all four interacted with RPS2 and not with a related resistance protein, RPM1. Three of the four interacted solely with N-terminal domains of RPS2 and not with the RPS2 LRR domain. Interactions were not impacted by RPS2 nucleotide binding site mutations. When disease resistance was tested in Arabidopsis, plant lines carrying T-DNA insertions that disrupt the genes for the ankyrin/tetratricopeptide repeat protein or the protein of unknown function exhibited reduced resistance to P. syringae pv. tomato strain DC3000 expressing avrRpt2. The RPS2 interacting proteins identified in this screen are new targets for studies of plant defense signal transduction. © 2005 Elsevier Ltd. All rights reserved.
Publication Source (Journal or Book title)
Physiological and Molecular Plant Pathology
First Page
257
Last Page
267
Recommended Citation
Quirino, B., Genger, R., Ham, J., Zabala, G., & Bent, A. (2004). Identification and functional analysis of Arabidopsis proteins that interact with resistance gene product RPS2 in yeast. Physiological and Molecular Plant Pathology, 65 (5), 257-267. https://doi.org/10.1016/j.pmpp.2005.02.006