Analysis Methods of Phenolic Acids

Document Type

Article

Publication Date

2-21-2012

Abstract

Phenolic acids are broadly distributed in the plant kingdom. Studies have shown that phenolic acids in fruits, vegetables, and grains have health benefits ofpreventing chronic diseases such as cardiovascular diseases, obesity, diabetes, and various cancers. High-pressure liquid chromatography (HPLC) is widely used for qualitative and quantitative analysis of phenolic acids. Due to the complexity of different sample matrices and bound phenolic acids, different sample preparation methods including acidic, basic and enzymatic hydrolysis, solvent extraction, and solid phase cartridge cleanup step are discussed in this chapter. Also, different mobile phase compositions are introduced for obtaining satisfactory separation and reduced analysis time based on the phenolic acids profile of different samples. In addition ultraviolet (UV) detection, HPLC with florescence (FL), electrochemical (EC), or mass spectrometric (MS) detection used for the analysis are discussed. Gas chromatography (GC) analysis of phenolic acids and sample derivatization are also included in this chapter. © 2012 John Wiley & Sons, Ltd.

Publication Source (Journal or Book title)

Analysis of Antioxidant-Rich Phytochemicals

First Page

69

Last Page

104

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