Optimizing time and temperature of enzymatic conversion of isoflavone glucosides to aglycones in soy germ flour

Document Type

Article

Publication Date

11-10-2010

Abstract

Five factors (enzyme concentration, substrate concentration, pH, incubation temperature, and incubation time) were initially screened for the conversion of isoflavone glucosides to aglycones in soy germ flour. The incubation temperature/time most significantly affected aglycone yield; subsequently, a full 5 (35, 40, 45, 50, and 55 °C) × 6 (1, 2, 3, 4, 5, and 6 h) factorial design and response surface methodology were employed to attain an optimal incubation time/temperature condition. The optimum condition producing soy germ flour with a high concentration of daidzein, glycitein, and genistein was as follows: soy germ flour:deionized water (1:5, w/v), β-glucosidase at 1 unit/g of soy germ flour, pH 5, and incubation temperature/time of 45 °C/5 h. Under this optimal condition, most isoflavone glucosides were converted to aglycones with daidzein, glycitein, and genistein of ≥15.4, ≥6.16, and ≥4.147 μmol/g, respectively. In contrast, the control soy germ flour contained 13.82 μmol/g daidzin, 7.11 μmol/g glycitin, 4.40 μmol/g genistin, 1.56 μmol/g daidzein, 0.52 μmol/g glycitein, and 0.46 μmol/g genistein. © 2010 American Chemical Society.

Publication Source (Journal or Book title)

Journal of Agricultural and Food Chemistry

First Page

11340

Last Page

11345

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