Detection of Antibody Specificity of Raw Bovine and Human Milk to Bacterial Lipopolysaccharides Using PCFIA

Document Type

Article

Publication Date

1-1-1993

Abstract

Raw milk from non-immunized cows and raw human milk from lactating mothers were examined for specificity and antibody activity against lipopolysaccharides (LPS) of five pathogenic bacteria, i.e. Escherichia coli O111:B4, E. coli 0128:B12, Salmonella enteritidis, S. typhimurium and Shigella flexneri 1A in a particle concentration fluorescence immunoassay (PCFIA). Bacterial LPS was covalently coated on submicron polystyrene particles and used in an antibody sandwich technique with commercially available rabbit anti-bovine fluorescein-labeled IgG or goat anti-human fluorescein-labeled IgA. Comparison was made to a skim milk sample obtained from vaccinated cows. In general, specific activity of milk IgG against bacterial LPS did not parallel the trend of total non-specific activity. Although the specific anti-LPS activities were in general high in milk from vaccinated cows, milk from non-immunized cows also showed significant activity against the bacterial LPS, sometimes higher than the vaccinated cows. IgA in human milk samples showed a wider range of antibody activity against the LPS fractions than bovine milk samples. The specificity of antibody from milk obtained from non-immunized cows against pathogenic bacteria demonstrated that immunization of cows in order to obtain milk with high titer antibody may not be necessary as milk from non-immunized cows can provide IgG with sufficient antibody activity against the LPS of bacterial pathogens. This can translate into significant savings and reduce stress imposed on animals for immunization. © 1993, Taylor & Francis Group, LLC. All rights reserved.

Publication Source (Journal or Book title)

Food and Agricultural Immunology

First Page

231

Last Page

239

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