Trans -Resveratrol inhibits hyperglycemia-induced inflammation and connexin downregulation in retinal pigment epithelial cells

Document Type

Article

Publication Date

7-28-2010

Abstract

The purpose of this study was to determine the inhibitory activity of trans-resveratrol against hyperglycemia-induced inflammation and degradation of gap junction intercellular communication in retinal pigment epithelial cells. Retinal (ARPE-19) cells were incubated with 5.5 mM glucose, 5.5 mM glucose and 10 μM resveratrol, 33 mM glucose, or 33 mM glucose and 0-10 μM trans-resveratrol at 37 °C and 5% CO2 for 9 days. Cell viability was determined by the crystal violet assay. The levels of low-grade inflammation biomarkers interleukin-6 and interleukin-8 (IL-6 and IL-8), angiogenic factors, and vascular endothelial growth factor (VEGF) were determined by the enzyme-linked immunosorbent assay (ELISA). Gap junction intercellular communication (GJIC) was determined by the scrape-load/dye transfer method. The expression levels of protein kinase Cβ (PKCβ), connexin 43 (Cx43), transforming growth factor-β1 (TGF-β1), and cyclooxygenase-2 (COX-2) were determined by Western blot. Incubation of retinal cells with 10 μM trans-resveratrol in the presence of 5.5 mM glucose did not affect any of the biomarkers investigated. Incubation of ARPE-19 cells with 33 mM glucose for 9 days significantly induced the accumulation of VEGF, IL-6, IL-8, TGF-β, and COX-2, activation of PKCβ, and reduction of Cx43 and GJIC. Incubation of ARPE-19 cells with 33 mM glucose in the presence of 0-10 μM trans-resveratrol dose-dependently inhibited VEGF, TGF-β1, COX-2, IL-6, and IL-8 accumulation, PKCβ activation, and Cx43 degradation and enhanced GJIC. These data suggest that trans-resveratrol can protect the retinal pigment epithelial cells against hyperglycemia-induced low-grade inflammation and GJIC degradation. © 2010 American Chemical Society.

Publication Source (Journal or Book title)

Journal of Agricultural and Food Chemistry

First Page

8246

Last Page

8252

This document is currently not available here.

Share

COinS