Development of water-soluble chitosan powder and its antimicrobial effect against inoculated Listeria innocua NRRL B-33016 on shrimp

Document Type

Article

Publication Date

3-1-2018

Abstract

The objectives of this study were to produce water-soluble chitosan (WSC) powder and to evaluate the effect of WSC on the survival of Listeria innocua inoculated onto shrimp surfaces. WSC powder was produced using a combination of enzymatic hydrolysis, ultrafiltration, and freeze drying. WSC solutions of 0.5, 1, 3, and 5 g/100 mL concentrations were prepared by dissolving the WSC powder in water. All concentrations of the WSC solutions showed anti-L. innocua activity in vitro, with the 5 g/100 mL WSC solution reducing initial L. innocua counts by 7.43 log CFU/mL. Freshly acquired shrimp were inoculated with L. innocua to an initial concentration of 7.65 log CFU/g. The inoculated shrimp samples were separately dipped in water (control) and in the WSC solutions for 1 min. After dipping in deionized water and 0.5, 1, 3, and 5 g/100 mL WSC solutions, the L. innocua counts were reduced by 1.50, 1.99, 2.25, 3.56, and 5.34 log CFU/g, respectively. For aerobic bacteria loads (8.43 log CFU/g) including L. innocua and natural shrimp microflora, dipping in deionized water and 0.5, 1, 3, and 5 g/100 mL WSC solution resulted in 0, 0.69, 3.21, 3.71, and 4.43 log CFU/g reductions, respectively. This study demonstrated that a low viscosity (0.01–0.29 Pa s) WSC solution could be used as an antimicrobial agent to reduce microbial loads on the surface of shrimp.

Publication Source (Journal or Book title)

Food Control

First Page

453

Last Page

458

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