Theoretical prediction of optimal cooling rates for human Adipose Derived Adult Stem (ADAS) cells

Document Type

Conference Proceeding

Publication Date

12-1-2005

Abstract

This study reports the use of a well established differential scanning calorimeter (DSC) technique to measure the dehydration response during freezing in Adipose Derived Adult Stem (ADAS) cells. Volumetric shrinkage during freezing of ADAS cells was obtained at a cooling rate of 20°C/min in the presence of extracellular ice and CPAs (10% DMSO and 10% Glycerol), ADAS cells from the stromal vascular fraction (SVF) and from two passages (Passage-0 (P0) and Passage-2 (P2)) were studied [1]. All passages and SVF cells were modeled as spheres with 50 urn diameter with an osmotically inactive volume, V b, of 0.6V0, where V o is the isotonic cell volume. A model of water transport was fit to the experimentally obtained volumetric shrinkage data to determine the membrane permeability parameters (L pg and E LP or L pg[CPA] and E Lp[CPA]). These biophysical parameters were then used to calculate the optimal cooling rates of ADAS cells in the presence and absence of CPAs.

Publication Source (Journal or Book title)

Proceedings of the 2005 Summer Bioengineering Conference

First Page

47

Last Page

48

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